Please use this identifier to cite or link to this item: http://docs.prosentient.com.au/prosentientjspui/handle/1/10178
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dc.contributor.authorYurick, David-
dc.contributor.authorKhoury, Georges-
dc.contributor.authorClemens, Bridie-
dc.contributor.authorLoh, Liyen-
dc.contributor.authorPham, Hai-
dc.contributor.authorKedzierska, Katherine-
dc.contributor.authorEinsiedel, Lloyd-
dc.contributor.authorPurcell, Damian-
dc.date2018-
dc.date.accessioned2019-01-17T23:08:35Z-
dc.date.accessioned2019-06-29T00:36:49Z-
dc.date.available2019-01-17T23:08:35Z-
dc.date.available2019-06-29T00:36:49Z-
dc.date.issued2018-12-05-
dc.identifier.citationJournal of clinical microbiology 2018-12-05-
dc.identifier.urihttp://docs.prosentient.com.au/prosentientjspui/handle/1/10178-
dc.description.abstractDuring human T-cell leukemia virus type-1 (HTLV-1) infection the frequency of cells harboring an integrated copy of viral cDNA, the proviral load (PVL), is the main risk factor for progression of HTLV-1-associated diseases. Accurate quantification of provirus by droplet digital PCR (ddPCR) is a powerful diagnostic tool with emerging uses for monitoring viral expression. Current ddPCR techniques quantify HTLV-1 PVL in terms of whole genomic cellular material, while the main target of HTLV-1 infection is the CD4+ and CD8+ T-cell. Our understanding of HTLV-1 proliferation and the amount of viral burden present in different compartments is limited. Recently a sensitive ddPCR assay was applied to quantifying T-cells by measuring loss of germline T-cell receptor genes as method of distinguishing non-T-cell from recombined T-cell DNA. In this study, we demonstrated and validated novel applications of the duplex ddPCR assay to quantify T-cells from various sources of human gDNA extracted from frozen material (PBMCs, bronchoalveolar lavage, and induced sputum) from a cohort of remote Indigenous Australians and then compared the T-cell measurements by ddPCR to the prevailing standard method of flow cytometry. The HTLV-1c PVL was then calculated in terms of extracted T-cell gDNA from various compartments. Because HTLV-1c preferentially infects CD4+ T-cells, and the amount of viral burden correlates with HTLV-1c disease pathogenesis, application of this ddPCR assay to accurately measure HTLV-1c-infected T-cells can be of greater importance for clinical diagnostics, prognostics as well as monitoring therapeutic applications.-
dc.language.isoeng-
dc.titleA Multiplex Droplet Digital PCR Assay for Quantification of HTLV-1c DNA Proviral Load and T-Cells from Blood and Respiratory Exudates Sampled in a Remote Setting.-
dc.typeJournal Article-
dc.identifier.doi10.1128/JCM.01063-18-
dc.identifier.journaltitleJournal of clinical microbiology-
dc.identifier.pubmedurihttps://www.ezpdhcs.nt.gov.au/login?url=https://www.ncbi.nlm.nih.gov/pubmed/30518541-
dc.identifier.pubmedidhttps://www.ezpdhcs.nt.gov.au/login?url=https://www.ncbi.nlm.nih.gov/pubmed/30518541-
dc.identifier.affiliationDepartment of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity at The University of Melbourne, Parkville, VIC 3010, Australia..-
dc.identifier.affiliationDepartment of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity at The University of Melbourne, Parkville, VIC 3010, Australia..-
dc.identifier.affiliationDepartment of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity at The University of Melbourne, Parkville, VIC 3010, Australia..-
dc.identifier.affiliationDepartment of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity at The University of Melbourne, Parkville, VIC 3010, Australia..-
dc.identifier.affiliationBaker Heart and Diabetes Institute, Alice Springs NT, Australia..-
dc.identifier.affiliationDepartment of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity at The University of Melbourne, Parkville, VIC 3010, Australia..-
dc.identifier.affiliationBaker Heart and Diabetes Institute, Alice Springs NT, Australia.. Department of Medicine, Alice Springs Hospital, Alice Springs NT, Australia..-
dc.identifier.affiliationDepartment of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity at The University of Melbourne, Parkville, VIC 3010, Australia..-
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