Please use this identifier to cite or link to this item: http://docs.prosentient.com.au/prosentientjspui/handle/1/10184
Title: Contaminated fingers: a potential cause of Chlamydia trachomatis-positive urine specimens.
Authors: Giffard, Philip M
Lilliebridge, Rachael A
Wilson, Judith
Murray, Gerald
Phillips, Samuel
Tabrizi, Sepehr N
Garland, Suzanne M
Martin, Louise
Singh, Gurmeet
Tong, Steven Y C
Holt, Deborah C
Andersson, Patiyan
Affiliation: Global and Tropical Health Division, Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia.. School of Psychological and Clinical Sciences, Charles Darwin University, Darwin, Northern Territory, Australia..
Global and Tropical Health Division, Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia..
Global and Tropical Health Division, Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia..
Murdoch Childrens Research Institute, Melbourne, Victoria, Australia.. Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, Melbourne, Victoria, Australia..
Murdoch Childrens Research Institute, Melbourne, Victoria, Australia.. Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, Melbourne, Victoria, Australia..
Murdoch Childrens Research Institute, Melbourne, Victoria, Australia.. Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, Melbourne, Victoria, Australia.. Department of Obstetrics and Gynaecology, University of Melbourne, Melbourne, Victoria, Australia.. Department of Microbiology, Royal Children's Hospital, Melbourne, Victoria, Australia..
Murdoch Childrens Research Institute, Melbourne, Victoria, Australia.. Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, Melbourne, Victoria, Australia.. Department of Obstetrics and Gynaecology, University of Melbourne, Melbourne, Victoria, Australia.. Department of Microbiology, Royal Children's Hospital, Melbourne, Victoria, Australia..
Royal Darwin Hospital, Top End Health Service, Northern Territory Government, Darwin, Northern Territory, Australia..
Royal Darwin Hospital, Top End Health Service, Northern Territory Government, Darwin, Northern Territory, Australia.. Sexual Assault Referral Centre, Northern Territory Government, Northern Territory, Australia.. Northern Territory Medical Program, Flinders University, Darwin, Northern Territory, Australia.. Child Health Division, Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia..
Global and Tropical Health Division, Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia.. Victorian Infectious Disease Service, The Royal Melbourne Hospital, and The University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia..
Global and Tropical Health Division, Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia.. School of Psychological and Clinical Sciences, Charles Darwin University, Darwin, Northern Territory, Australia..
Global and Tropical Health Division, Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia..
Issue Date: Feb-2018
Citation: Sexually transmitted infections 2018-02; 94(1): 32-36
Abstract: The detection of an STI agent in a urogenital tract (UGT) specimen from a young child is regarded as being indicative of sexual abuse. However, the probabilities of contamination events that could conceivably lead to STI positive specimens in the absence of sexual contact are unclear. The objective was to estimate the potential for fingers that have come in contact with Chlamydia trachomatis-positive urine to detectably contaminate C. trachomatis-negative urine. The study design was based on self-experimentation. Dilutions of C. trachomatis elementary bodies (EBs) were prepared. A participant contacted an EB dilution then a urine surrogate specimen. The experiment was performed by three participants using three C. trachomatis isolates, of genotype E, F and B. Two surrogate urine contact methods were used to mimic contamination of a carer assisting with a child's urine collection. All EB dilutions and urine surrogate specimens were subjected to C. trachomatis assay and quantification in a real-time PCR-based diagnostic system. The amplimer crossing point (Cq) for EB dilutions was 10.0±1.6 less than for corresponding finger contacted urine specimens, which corresponds to ~10 µL of EB suspension transferred. This was largely independent of participant identity, C. trachomatis strain or EB dilution. Hand decontamination led to large reductions in EBs transferred, but transfer remained consistently detectable. Recent Cq data from C. trachomatis-positive clinical urine specimens were collated, and 20% clearly contained sufficient C. trachomatis to detectably contaminate another specimen by finger-mediated transfer, as in this experiment. This study directly demonstrated the potential for urine contaminated fingers to convert a C. trachomatis-negative urine specimen to C. trachomatis positive as a result of contact. Accordingly, procedures for urine specimen collection, particularly from children, need to be designed to prevent contamination.
URI: http://docs.prosentient.com.au/prosentientjspui/handle/1/10184
DOI: 10.1136/sextrans-2016-053081
Type: Journal Article
Subjects: children
chlamydia trachomatis
sexual abuse
urine
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