Please use this identifier to cite or link to this item: http://docs.prosentient.com.au/prosentientjspui/handle/1/10422
Title: Next Generation Diagnostics for Melioidosis: Evaluation of a Prototype i-STAT Cartridge to Detect Burkholderia pseudomallei Biomarkers.
Authors: Schully, Kevin L
Young, Charles C
Mayo, Mark
Connolly, Amy L
Rigas, Vanessa
Spall, Ammarah
Chan, Alyssa A
Salvador, Mark G
Lawler, James V
Opdyke, Jason A
Clark, Danielle V
Currie, Bart J
Affiliation: Austere environments Consortium for Enhanced Sepsis Outcomes (ACESO), Biological Defense Research Directorate, Naval Medical Research Center-Frederick, Ft. Detrick, MD, USA..
The Johns Hopkins University Applied Physics Laboratory, Laurel, MD, USA..
Global and Tropical Health Division, Menzies School of Health Research, Darwin, Australia..
The Johns Hopkins University Applied Physics Laboratory, Laurel, MD, USA..
Global and Tropical Health Division, Menzies School of Health Research, Darwin, Australia..
Austere environments Consortium for Enhanced Sepsis Outcomes (ACESO), Biological Defense Research Directorate, Naval Medical Research Center-Frederick, Ft. Detrick, MD, USA..
Austere environments Consortium for Enhanced Sepsis Outcomes (ACESO), Biological Defense Research Directorate, Naval Medical Research Center-Frederick, Ft. Detrick, MD, USA..
Austere environments Consortium for Enhanced Sepsis Outcomes (ACESO), Biological Defense Research Directorate, Naval Medical Research Center-Frederick, Ft. Detrick, MD, USA..
Austere environments Consortium for Enhanced Sepsis Outcomes (ACESO), Biological Defense Research Directorate, Naval Medical Research Center-Frederick, Ft. Detrick, MD, USA..
Joint Program Executive Office for Chemical, Biological, Radiological and Nuclear Defense, Medical Countermeasure Systems, Ft. Detrick, MD, USA..
Austere environments Consortium for Enhanced Sepsis Outcomes (ACESO), Biological Defense Research Directorate, Naval Medical Research Center-Frederick, Ft. Detrick, MD, USA..
Global and Tropical Health Division, Menzies School of Health Research, Darwin, Australia.. Department of Infectious Diseases and Northern Territory Medical Program, Royal Darwin Hospital, Darwin, Australia..
Issue Date: 31-Oct-2018
Citation: Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 2018-10-31
Abstract: Infection with the gram-negative bacterium Burkholderia pseudomallei can result in melioidosis, a life-threatening disease that can be difficult to diagnose. Culture remains the gold standard for diagnosis but requires laboratory resources not available in many endemic regions. A lateral flow immunoassay has shown promise for POC diagnostics but suffers from low sensitivity when used on blood samples. PCR also has low sensitivity on blood, attributed to the low bacterial numbers in blood observed in melioidosis patients, even when bacteraemic. A prototype i-STAT cartridge was developed to utilize the monoclonal antibody specific for the capsule of pathogenic Burkholderia species employed on the LFI. The resulting POC assay was evaluated on 414 clinical specimens from Darwin, Australia and Cambodia. The i-STAT assay accurately distinguished Australian blood culture positive melioidosis patients from Australian patients hospitalized with other infections (AUC = 0.91, 95% CI 0.817 - 1.0). We derived an assay cutoff with 76% sensitivity and 94% specificity that correctly classified 88% (n=74) of the Australian patients. Interestingly, only 46% (6/13) of the culture-positive melioidosis patients in Cambodia were classified correctly. Of great importance however, the assay detected capsule from blood samples for 32% of blood culture negative melioidosis patients in both cohorts and previously undiagnosed melioidosis patients in Cambodia. In addition the assay showed high sensitivity and specificity for urine, pus and sputum. Diagnostic tools that are not dependent upon the growth kinetics or the levels of bacteremia of B. pseudomallei represent the next-generation of diagnostics and must be pursued further.
URI: http://docs.prosentient.com.au/prosentientjspui/handle/1/10422
DOI: 10.1093/cid/ciy929
Type: Journal Article
Appears in Collections:NT Health digital library

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