Please use this identifier to cite or link to this item: http://docs.prosentient.com.au/prosentientjspui/handle/1/10538
Title: Multitarget PCR Assay for Direct Detection of Penicillinase-Producing Neisseria gonorrhoeae for Enhanced Surveillance of Gonococcal Antimicrobial Resistance.
Authors: Buckley, Cameron
Trembizki, Ella
Baird, Robert W
Chen, Marcus
Donovan, Basil
Freeman, Kevin
Goire, Namraj
Guy, Rebecca
Lahra, Monica M
Regan, David
Whiley, David M
Affiliation: Queensland Paediatric Infectious Diseases Laboratory, Queensland Children's Medical Research Institute, Queensland, Australia The University of Queensland, Brisbane, Queensland, Australia..
Queensland Paediatric Infectious Diseases Laboratory, Queensland Children's Medical Research Institute, Queensland, Australia The University of Queensland, Brisbane, Queensland, Australia..
Microbiology Laboratory, Pathology Department, Royal Darwin Hospital, Darwin, Northern Territory, Australia..
Melbourne Sexual Health Centre, Alfred Health, Carlton Victoria, Australia Central Clinical School, Monash University, Victoria, Australia..
Kirby Institute, University of New South Wales, Sydney, NSW, Australia Sydney Sexual Health Centre, Sydney Hospital, Sydney, NSW, Australia..
Microbiology Laboratory, Pathology Department, Royal Darwin Hospital, Darwin, Northern Territory, Australia..
WHO Collaborating Centre for STD, Microbiology Department, South Eastern Area Laboratory Services, Prince of Wales Hospital, Sydney, New South Wales, Australia Nepean Clinical School, University of Sydney, Kingswood, New South Wales, Australia..
Kirby Institute, University of New South Wales, Sydney, NSW, Australia..
WHO Collaborating Centre for STD, Microbiology Department, South Eastern Area Laboratory Services, Prince of Wales Hospital, Sydney, New South Wales, Australia..
Kirby Institute, University of New South Wales, Sydney, NSW, Australia..
Queensland Paediatric Infectious Diseases Laboratory, Queensland Children's Medical Research Institute, Queensland, Australia The University of Queensland, Brisbane, Queensland, Australia d.whiley@uq.edu.au..
Issue Date: Aug-2015
Citation: Journal of clinical microbiology 2015-08; 53(8): 2706-8
Abstract: A multitarget PCR was developed for the direct detection of penicillinase-producing Neisseria gonorrhoeae (PPNG). The assay was validated by testing 342 PPNG isolates and 415 clinical samples. The method is suitable for routine detection of PPNG strains. Its multitarget approach reduces the potential for false-negative results caused by sequence variations.
URI: http://docs.prosentient.com.au/prosentientjspui/handle/1/10538
DOI: 10.1128/JCM.00540-15
Type: Evaluation Studies
Journal Article
Research Support, Non-U.S. Gov't
Subjects: Epidemiological Monitoring
Gonorrhea
Humans
Neisseria gonorrhoeae
Penicillinase
Polymerase Chain Reaction
Drug Resistance, Bacterial
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