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|Title:||Evaluation of PCR for diagnosis of melioidosis.|
|Affiliation:||Royal Darwin Hospital, Tiwi, Northern Territory, Australia. email@example.com.|
|Citation:||Journal of clinical microbiology 1998-04; 36(4): 1039-41|
|Abstract:||Previously published PCR-based diagnostic tests for melioidosis were evaluated for clinical usefulness. A Burkholderia pseudomallei 16S rRNA-derived primer set had a sensitivity approaching 100% for clinical samples from 22 culture-confirmed cases of melioidosis and enabled diagnosis of 3 culture-negative cases. However, samples from 10 of 30 inpatients from Royal Darwin Hospital with other diagnoses were positive by PCR, giving a specificity of 67% and a positive predictive value of only 70%. Although there are a number of intriguing possible explanations for our results, concerns of inappropriate therapy resulting from a positive result by PCR have led us to forgo the advantage of rapid PCR diagnosis for melioidosis until a better system is validated.|
Research Support, Non-U.S. Gov't
RNA, Ribosomal, 16S
Polymerase Chain Reaction
|Appears in Collections:||NT Health digital library|
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