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Title: Pseudomonas pseudomallei isolates collected over 25 years from a non-tropical endemic focus show clonality on the basis of ribotyping.
Authors: Currie, B
Smith-Vaughan, H
Golledge, C
Buller, N
Sriprakash, K S
Kemp, D J
Affiliation: Menzies School of Health Research and Royal Darwin Hospital, Australia..
Issue Date: Oct-1994
Citation: Epidemiology and infection 1994-10; 113(2): 307-12
Abstract: Between 1966 and 1991, melioidosis, a disease caused by Pseudomonas pseudomallei that is mostly confined to tropical regions, occurred in farm animals and a farmer in temperate south-west Western Australia. Using an Escherichia coli probe containing a ribosomal RNA operon, P. pseudomallei DNA from isolates from 8 animals, a soil sample and the human case showed an identical ribotype on Southern blotting. The ribotype was different from the 3 commonest ribotypes seen in tropical Australia. This molecular typing supports the theory of clonal introduction of P. pseudomallei into a non-endemic region, with environmental contamination, local dissemination and persistence over 25 years. As melioidosis is often fatal in humans, such persistence in a temperate region is cause for concern.
ISSN: 0950-2688
Type: Journal Article
Subjects: Animals
Bacterial Typing Techniques
Blotting, Southern
Burkholderia pseudomallei
DNA Probes
Electrophoresis, Agar Gel
Western Australia
rRNA Operon
Appears in Collections:NT Health digital library

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