Please use this identifier to cite or link to this item: http://docs.prosentient.com.au/prosentientjspui/handle/1/11150
Title: The microbiome of otitis media with effusion in Indigenous Australian children.
Authors: Jervis-Bardy, Jake
Rogers, Geraint B
Morris, Peter S
Smith-Vaughan, Heidi C
Nosworthy, Elizabeth
Leong, Lex E X
Smith, Renee J
Weyrich, Laura S
De Haan, Jacques
Carney, A Simon
Leach, Amanda J
O'Leary, Stephen
Marsh, Robyn L
Affiliation: Child Health Division, Menzies School of Health Research, Charles Darwin University, Darwin, NT, Australia; School of Medicine, Flinders University, Adelaide, SA, Australia..
Infection and Immunity Theme, South Australia Health and Medical Research Institute, Adelaide, SA, Australia..
Child Health Division, Menzies School of Health Research, Charles Darwin University, Darwin, NT, Australia..
Child Health Division, Menzies School of Health Research, Charles Darwin University, Darwin, NT, Australia..
Child Health Division, Menzies School of Health Research, Charles Darwin University, Darwin, NT, Australia..
Infection and Immunity Theme, South Australia Health and Medical Research Institute, Adelaide, SA, Australia..
Infection and Immunity Theme, South Australia Health and Medical Research Institute, Adelaide, SA, Australia; School of Biological Sciences, Flinders University, Adelaide, SA, Australia..
Australian Centre for Ancient DNA, University of Adelaide, Adelaide, SA, Australia..
Department of Otolaryngology, Alice Springs Hospital, Alice Springs, NT, Australia..
Department of Otolaryngology-Head & Neck Surgery, Flinders University, Adelaide, SA, Australia..
Child Health Division, Menzies School of Health Research, Charles Darwin University, Darwin, NT, Australia..
Department of Otolaryngology, University of Melbourne, Melbourne, VIC, Australia..
Child Health Division, Menzies School of Health Research, Charles Darwin University, Darwin, NT, Australia. Electronic address: robyn.marsh@menzies.edu.au..
Issue Date: Sep-2015
Citation: International journal of pediatric otorhinolaryngology 2015-09; 79(9): 1548-55
Abstract: Indigenous Australian children have a high prevalence of otitis media with effusion (OME) and associated conductive hearing loss. Only three microbiological studies of middle ear fluid (MEF) from Indigenous Australian children with OME have been reported. All of these were reliant on culture or species-specific PCR assays. The aim of this study was to characterise the middle ear fluid (MEF), adenoid and nasopharyngeal (NP) microbiomes of Indigenous Australian children, using culture-independent 16S rRNA gene sequencing. MEF, NP swabs and adenoid specimens were collected from 11 children in the Alice Springs region of Central Australia. Bacterial communities in these specimens were characterised using 16S rRNA gene sequencing. The microbiota in MEF samples were dominated (>50% relative abundance) by operational taxonomic units (OTUs) consistent with Alloiococcus otitidis (6/11), Haemophilus influenzae (3/11) or Streptococcus sp. (specifically, Mitis group streptococci which includes Streptococcus pneumoniae) (1/11). Anatomical site selectivity was indicated by the presence of a single conserved Haemophilus OTU in 7/11 MEF samples. In comparison, there were ten distinct Haemophilus OTUs observed across the NP and adenoid samples. Despite significant differences between the MEF and NP/adenoid microbiomes, Streptococcus sp., H. influenzae and Moraxella catarrhalis OTUs were common to all sample types. Co-occurrence of classical otopathogens in paired MEF and NP/Adenoid samples is consistent with earlier culture-based studies. These data highlight the need to further assess H. influenzae traits important in otitis media and to understand the role of canal flora, especially A. otitidis, in populations with a high prevalence of tympanic membrane perforation.
URI: http://docs.prosentient.com.au/prosentientjspui/handle/1/11150
DOI: 10.1016/j.ijporl.2015.07.013
Type: Journal Article
Research Support, Non-U.S. Gov't
Subjects: 16S ribosomal RNA
Indigenous
Microbiome
Middle ear effusion
Otitis media
Adenoids
Australia
Carnobacteriaceae
Child
Child, Preschool
Ear, Middle
Female
Haemophilus influenzae
Humans
Male
Microbiota
Moraxella (Branhamella) catarrhalis
Nasopharynx
Oceanic Ancestry Group
Otitis Media with Effusion
RNA, Bacterial
RNA, Ribosomal, 16S
Streptococcus
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