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Title: Polymerase chain reaction based diagnostic to identify larvae and eggs of container mosquito species from the Australian region
Authors: Beebe, Nigel W
Cooper, Robert D
Corcoran, Sarah
Ritchie, Scott A
van den Hurk, Andrew
Whelan, Peter I
Issue Date: Mar-2007
Publisher: Entomological Society of America
Series/Report no.: Journal of Medical Entomology
Vol. 44, No. 2
Abstract: Dengue outbreaks occur regularly in parts of northern Queensland, Australia, and there is concern that these outbreaks may spread with the introduction and range expansion of the two main vectors Aedes aegypti and Aedes albopictus (Skuse). Problems encountered in separating larvae of endemic and exotic container mosquito species resulted in the development of a polymerase chain reaction diagnostic procedure that uses a restriction enzyme to cut the internal transcribed spacer region 1 of the ribosomal DNA to separate Ae. aegypti and Ae. albopictus from a number of common local container mosquito species which can be used at any stage of the life cycle, including eggs up to 8 wk of age. Identification was possible using desiccated or alcohol-preserved specimens with a response time of <24 h after receipt of the specimens.
ISSN: 0022-2585
Type: Journal Article
Subjects: Insects
Diagnostic tests
Genetic techniques
Appears in Collections:NT General Collection

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